Hep Par 1, Hepatocyte Paraffin 1

Hep Par 1 is a monoclonal antibody, clone OCH1E5.2.10. The antigen detected by Hep Par 1 does not appear to be an oncofetal antigen or tumour-associated antigen. There is preliminary evidence that it is associated with the membrane of hepatocyte mitochondria2 but absent from the mitochondria of other tissues.

The antibody is effective on formalin-fixed, paraffin embedded tissues. Antigen retrieval using high pH buffer and a detection kit which minimizes or eliminates non-specific biotin reactivity is recommended4. Staining is cytoplasmic and granular, without canalicular or zonal accentuation4.

Immunohistochemical expression

 

columnar lined oesophagus without intestinal metaplasia

0/216

 

columnar lined oesophagus with intestinal metaplasia

31/3116

chronic gastritis with intestinal metaplasia

13/1316

   
   
 

hepatocellular carcinoma

30/321, 37/382, 289/2903, 17/215, 9/106, 12/127, 33/408, 6/910, 18/1911 , 39/4212, 88/9613, 50/7615

hepatoblastoma

12/1214, 1/15

metastatic adenocarcinoma, NOS

0/131, 1/185, 0/75 15

biliary tract carcinoma

4/321, 2/352, 3/65, 0/38, 1/1413

mixed hepatocellular carcinoma / cholangiocarcinoma

0/35

pancreatic carcinoma

3/122, 0/88, 1/1313

breast carcinoma

0/92, 0/68, 0/913

oesophageal adenocarcinoma

1/916

gastric carcinoma

3/102, 10/1012, 4/1313, 1/815, 4/1316

small intestinal carcinoma

0/62

colon carcinoma

0/82, 1/108, 8/10411, 0/1013

lung carcinoma

0/52, 1/108, 3/5511, 0/612, 5/2113

endometrial carcinoma

0/32, 0/1013

ovarian carcinoma

0/72, 5/4011, 4/2413

uterine cervix

1/511

prostatic carcinoma

0/42, 0/713

renal carcinoma

0/52, 0/106, 0/512, 0/1013, 0/7415

hepatoid adenocarcinoma

6/79, 2/610

hepatoid yolk sac tumour

4/810

adrenocortical carcinoma

3/1311, 0/812, 0/1013, 0/2115

small cell carcinoma of lung and skin

0/1513

salivary gland

0/1913

germ cell

0/1413

yolk sac tumour

2/911

urinary bladder

0/12, 1/1012, 0/1513

vascular liver tumour

0/52

melanoma

0/32

mesothelioma

0/32, 0/1613

neuroendocrine carcinoma

0/142, 0/135, 4/913

carcinoid

1/1013

phaeochromocytoma

0/22

testicular tumours

0/22

skin, basal cell carcinoma

0/1013

skin, Merkel cell carcinoma

0/913

skin, squamous cell carcinoma

0/1013

epithelioid sarcoma

0/1013

thymoma

0/813

thyroid

0/1413

   
 

Diagnostic utility

References

1 Leong, A. S., Sormunen, R. T., Tsui, W. M., Liew, C. T. Hep Par 1 and selected antibodies in the immunohistological distinction of hepatocellular carcinoma from cholangiocarcinoma, combined tumours and metastatic carcinoma. Histopathology 1998;33:318-324.

2 Wennerberg, A. E., Nalesnik, M. A., Coleman, W. B. Hepatocyte paraffin 1: a monoclonal antibody that reacts with hepatocytes and can be used for differential diagnosis of hepatic tumors. Am J Pathol 1993;143;1050-4.

3 Wu, P. C., Fang, J. W., Lau, V. K., Lai, C. L., Lo, C. K., Lau, J. Y. Classification of hepatocellular carcinoma according to hepatocellular and biliary differentiation markers. Clinical and biological implications. Am J Pathol 1996;149:1167-75.

4 Lamps, L.W. and Folpe, A.L. The diagnostic value of hepatocyte paraffin antibody 1 in differentiating hepatocellular neoplasms from nonhepatic tumors: a review. Adv Anat Pathol 2003;10:39-43.

5 Minervini, M.I., Demetris, A.J., Lee, R.G., Carr, B.I., Madariaga, J. and Nalesnik, M.A. Utilization of hepatocyte-specific antibody in the immunocytochemical evaluation of liver tumors. Mod Pathol 1997;10:686-92.

6 Murakata, L.A., Ishak, K.G. and Nzeako, U.C. Clear cell carcinoma of the liver: a comparative immunohistochemical study with renal clear cell carcinoma. Mod Pathol 2000;13:874-81.

7 Kumagai I et al. Immunoreactivity to monoclonal antibody, Hep Par 1, in human hepatocellular carcinomas according to histopathological grade and histologic pattern. Hepatology Research 2001;20:312-319.

8 Zimmerman, R.L., Burke, M.A., Young, N.A., Solomides, C.C. and Bibbo, M. Diagnostic value of hepatocyte paraffin 1 antibody to discriminate hepatocellular carcinoma from metastatic carcinoma in fine-needle aspiration biopsies of the liver. Cancer 2001;93:288-91.

9 Maitra, A., Murakata, L.A. and Albores-Saavedra, J. Immunoreactivity for hepatocyte paraffin 1 antibody in hepatoid adenocarcinomas of the gastrointestinal tract. Am J Clin Pathol 2001;115:689-94.

10 Pitman M et al. Hepatocyte paraffin 1 antibody cannot distinguish primary ovarian malignancies with hepatoid differentiation from metastatic hepatocellular carcinoma. Mod Pathol 2002;15:206A. (abstract)

11 Fan Z et al. HepPar-1 stain for the differential diagnosis of hepatocellular carcinoma: 613 tumors tested using tissue microarrays and conventional sections. Mod Pathol 2002;15:1178A. (abstract)

12 Muir T et al. Immunoreacitivity for hepatocyte paraffin 1 antibody in hepatic and extrahepatic tumors. Mod Pathol 2002;15:290A. (abstract)

13 Chu, P.G., Ishizawa, S., Wu, E. and Weiss, L.M. Hepatocyte antigen as a marker of hepatocellular carcinoma: an immunohistochemical comparison to carcinoembryonic antigen, CD10, and alpha-fetoprotein. Am J Surg Pathol 2002;26:978-88.

14 Fasano, M., Theise, N.D., Nalesnik, M., Goswami, S., Garcia de Davila, M.T., Finegold, M.J. and Greco, M.A. Immunohistochemical evaluation of hepatoblastomas with use of the hepatocyte-specific marker, hepatocyte paraffin 1, and the polyclonal anti-carcinoembryonic antigen. Mod Pathol 1998;11:934-8.

15 Wieczorek, T. J., J. L. Pinkus, et al. (2002). "Comparison of thyroid transcription factor-1 and hepatocyte antigen immunohistochemical analysis in the differential diagnosis of hepatocellular carcinoma, metastatic adenocarcinoma, renal cell carcinoma, and adrenal cortical carcinoma." Am J Clin Pathol 118(6): 911-21.

16 Chu, P. G., Z. Jiang, et al. (2003). "Hepatocyte antigen as a marker of intestinal metaplasia." Am J Surg Pathol 27(7): 952-9.

This page last revised 13.1.2004.

©SMUHT/PW Bishop