MPT64
The ZN tinctorial stain has a low sensitivity for acid fast bacilli. Immunohistochemistry using antiBCG also lacks sensitivity. A polyclonal rabbit antibody against the MPT64 mycobacterial antigen has a promise of greater sensitivity1. Specificity is improved by absorbing the antiserum against a strain of BCG that does not produce MPT64. In this study, cases of proven and suspected tuberculous lymphadenitis and various non-tuberculous lymph nodes were tested. There was a good correlation for positivity by immunohistochemistry and by PCR1. The antibody used was prepared in house.
|
Reference 1
|
PCR
|
|
MPT64
|
|
positive
|
negative
|
|
|
positive
|
36
|
6
|
42
|
|
negative
|
4
|
29
|
33
|
|
|
40
|
35
|
75
|
Diagnostic utility
Diagnosis of tuberculosis.
References
1 Mustafa T, Wiker HG, Mfinanga SG, et al. Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis. Mod Pathol 2006; 19:1606-14
This page last revised 7.1.2007.
©SMUHT/PW Bishop