| |
|
immunohistochemistry
for Merkel cell polyomavirus (against the MCV T antigen exon 2) |
quantitative
PCR for Merkel cell polyomavirus using T primer sets amplifying the
MCV
T antigen promoter region |
CK20 |
|
| Primary cutaneous Merkel cell carcinoma |
5/8 (all
positive cases were also positive by PCR)1 |
8/9 (The
negative case was a combined neuroendocrine and squamous cell
carcinoma) 1
|
8/81
|
| Merkel cell carcinoma metastatic to lymph node |
5/7 (all
positive cases were also positive by PCR)1 |
7/8 (The
one negative case was typical of Merkel cell carcinoma, positive
for CK20, but without a known cutaneous primary) 1 |
6/71
|
| Primary cutaneous Merkel cell carcinoma microarray |
12/141
|
|
13/141
|
Merkel cell carcinoma metastatic to lymph node
microarray |
15/221
|
|
19/221
|
Combined squamous and neuroendocrine carcinomas of the skin |
0/7 (negativity
in both components)1
|
|
7/7 (positivity
in the neuroendocrine component)1
|
| Primary
pulmonary small cell carcinoma (All primary pulmonary tumours had
been previously characterized by a panel of immunomarkers for
clinical purposes and found to be positive for at least one neuroendocrine
marker (chromogranin, synaptophysin, CD56). IHC for TTF-1 was
performed on 21 of the 26 tumors. Sixteen of them (76%) were immunoreactive
for TTF-1.) |
0/161
|
|
0/161
|
Primary
pulmonary large cell carcinoma (All primary pulmonary tumours had
been previously characterized by a panel of immunomarkers for
clinical purposes and found to be positive for at least one neuroendocrine
marker (chromogranin, synaptophysin, CD56). IHC for TTF-1 was
performed on 21 of the 26 tumors. Sixteen of them (76%) were immunoreactive
for TTF-1.)
|
0/101
|
|
0/101
|
Primary
parotid high-grade neuroendocrine carcinoma |
0/73 |
0/73 |
5/73 |
| |
|
|
|
A study by PCR of visceral high grade neuroendocrine tumours did not
detect Merkel cell polyomavirus in any of 73 cases2.