NB84
NB84 is an antibody raised against an antigen from human neuroblastoma tissue. It recognises an uncharacterised 57 kD molecule. It works reliably on formalin-fixed paraffin embedded tissue.
NB84 appears to be more sensitive but less specific than synaptophysin for neuroblastic differentiation2. Evaluation in decalcified bone trephines may be difficult due to diffuse intense background staining2.
Immunohistochemical expression
Non-neoplastic tissues showing positivity include some epithelia (tonsillar, colonic, pneumocytes, hepatocytes, renal, prostatic, basal cells of squamous epithelium), cardiac and skeletal muscle, brain, adrenal cortex and placental trophoblast2.
| |
Differentiated neuroblastoma
|
83/83(all tumours were negative for CD99. The stroma-rich cases showed fine punctate positivity of schwannian and ganglioneuroma-lien areas while ganglion cells and neuropil were strongly positive.)2
|
17/19(only 8/19 stained for neurofilament)1
|
|
| |
Undifferentiated neuroblastoma
|
21/22(all tumours were negative for CD99. 15 were synaptophysin positive,. All were neurofilament negative.)2
|
|
| |
Ewing's sarcoma
|
9/14(staining was weak and focal in most cases)1,
7/39(2 of 16 skeletal and 5 of 23 extra-skeletal)2
|
|
| |
PNET
|
4/92,
1/1(this case was negative for neurofilament)1
|
|
| |
Cerebellar medulloblastoma
|
3/182
|
|
| |
DSRCT
|
3/6(the numbers of positive cells are often less than in neuroblastoma)2
|
|
| |
Alveolar rhabdomyosarcoma
|
0/162
|
0/121
|
|
| |
Embryonal rhabdomyosarcoma
|
0/262
|
|
| |
Wilm's tumour
|
1/14(focal positivity in one case)2,
0/17(some of the differentiated tubular structures stained weakly)1
|
|
| |
Lymphoblastic lymphoma / ALL
|
0/122, 0/31
|
|
| |
Small cell carcinoma of lung
|
0/102
|
|
|
| |
|
|
|
|
| |
|
|
|
|
NB84 was more sensitive but less specific than synaptophysin2.
Diagnostic utility
References
1Thomas, J. O., J. Nijjar, et al. (1991). "NB84: a new monoclonal antibody for the recognition of neuroblastoma in routinely processed material." J Pathol 163(1): 69-75.
2Miettinen, M., J. Chatten, et al. (1998). "Monoclonal antibody NB84 in the differential diagnosis of neuroblastoma and other small round cell tumors." Am J Surg Pathol 22(3): 327-32.
This page last revised 15.1.2004.
©SMUHT/PW Bishop