Kappa/lambda ISH

In situ hybridisation for immunoglobulin light chains has advantages over immunohistochemistry in that it does not have the propensity to be confounded by background staining. Colorimetric ISH (CISH) can be automated1.

Diagnostic utility

Demonstration of monoclonality in B-cell proliferations, most often useful in myeloma and cases with plasmacytic differentiation.


1 Beck RC, Tubbs RR, Hussein M, et al. Automated colorimetric in situ hybridization (CISH) detection of immunoglobulin (Ig) light chain mRNA expression in plasma cell (PC) dyscrasias and non-Hodgkin lymphoma. Diagn Mol Pathol 2003; 12:14-20

This page last revised 18.11.2006.

©SMUHT/PW Bishop