PNL2

This monoclonal antibody was originally generated against the human somatostatin receptor but shows no reactivity with the immunizing antigen. Rather, it appears to be a useful melanocytic marker, although the antigen has not yet been identified.

Immunohistochemical expression

Normal tissues are negative, apart from neutrophils and cutaneous melanocytes1.

 

Compound naevus

15/151

staining most prominent in junctional and superficial dermal melanocytes, similar to the pattern with HMB45

  

Dysplastic naevus

15/151

 

Blue naevus

2/21

homogenous diffuse positivity
     
 

 

PNL2

Melan-A

HMB45

Tyrosinase

MitF

positive for all markers

negative for all markers

positive ONLY for PNL2

  

Primary epithelioid melanoma

13/131

 

 

 

 

 

 

 

Metastatic melanoma

33/381

31/381

29/381

35/381

32/381

26/381

1/381

2/381

Desmoplastic melanoma

1/131

1/131

1/131

0/131

1/131

 

 

 

Non-desmoplastic spindle cell melanoma

4/51

 

 

 

 

 

 

 

Angiomyolipoma

10/101

18/182

18/182

1/153

15/153

 

 

 
                 

Staining is strongest in epithelioid melanomas1.

 

Lung

squamous carcinoma

0/11

  

adenocarcinoma

0/11

Breast

ductal carcinoma

0/51

lobular carcinoma

0/51

Colon, adenocarcinoma

0/11

Renal clear cell carcinoma

0/51

Ovarian carcinoma

0/51

Transitional cell carcinoma

0/51

Fibrosarcoma

0/41

Leiomyosarcoma

0/31

Liposarcoma

0/51

Cellular dermatofibroma

0/51

Neurofibroma

0/51

Atypical fibroxanthoma

0/21

Cellular neurothekeoma

0/21

Granular cell tumour

0/11

MPNST

1/131

Melanotic Schwannoma

1/11

AML

0/51

CML

3/31
     
     
     
     
     
     
     
     
     
     
     

Diagnostic utility

References

1Busam KJ, Kucukgol D, Sato E, Frosina D, Teruya-Feldstein J,Jungbluth AA Immunohistochemical Analysis of Novel Monoclonal Antibody PNL2 and Comparison With Other Melanocyte Differentiation Markers. Am J Surg Pathol 2005; 29:400-406: this study was largely based on tissue micro-arrays.

2Jungbluth AA, Iversen K, Coplan K, et al. Expression of melanocyte-associated markers gp-100 and Melan-A/MART-1 in angiomyolipomas. An immunohistochemical and rt-PCR analysis. Virchows Arch 1999; 434:429-35

3Jungbluth AA, King R, Fisher DE, et al. Immunohistochemical and reverse transcription-polymerase chain reaction expression analysis of tyrosinase and microphthalmia-associated transcription factor in angiomyolipomas. Appl Immunohistochem Mol Morphol 2001; 9:29-34

This page last revised 15.3.2005.

©SMUHT/PW Bishop