This mixture of two antibodies works on formalin-fixed, paraffin embedded tissue.

Immunohistochemical expression

AE1 reacts with most acidic (type I) cytokeratins (K10, 15, 16 and 19) while AE3 recognises all known basic (type II) cytokeratins.

This mixture of antibodies reacts with almost all epithelia without showing cross-reactivity with other intermediate filaments.

Diagnostic utility


1Lerwill, M. F. (2004). "Current practical applications of diagnostic immunohistochemistry in breast pathology." Am J Surg Pathol 28(8): 1076-91.


©SMUHT/PW Bishop