Coordinate expression of cytokeratins 7 and 20

Predominantly CK7+/CK20-

CK7+/CK20+

CK7+/CK20-

CK7-/CK20+

CK7-/CK20-

Lung, adenocarcinoma

14% (6/541, 6/74(Tumours were considered positive if at least 10% of cells showed at least moderate immunoreactivity.)2, 2/155, 2/438, 1/1010, 1/2122, 18/4224)

76% (39/541, 64/74(Tumours were considered positive if at least 10% of cells showed at least moderate immunoreactivity.)2, 12/155, 16/17(signet ring cell carcinomas)6, 41/438, 9/1010, 20/2122, 14/16(using cell blocks)23, 24/4224, 30/4025)

0/541, 0/74(Tumours were considered positive if at least 10% of cells showed at least moderate immunoreactivity.)2, 0/155, 0/1010

10% (9/541, 4/74((Tumours were considered positive if at least 10% of cells showed at least moderate immunoreactivity.))2, 1/155, 0/1010, 2/16(using cell blocks)23, 10/4025)

Lung, bronchoalveolar

0/31, 8/28(7 mucinous and 1 mixed)8

3/31, 20/28(1 mucinous, 4 mixed and 15 non-mucinous)8

0/31

0/31

Malignant mesothelioma

0/161, 0/1710

11/161, 11/1710

0/161, 0/1710

5/161, 6/1710

Breast, infiltrating ductal

6/381, 0/2010

31/381, 19/2010, 6/6(tumour type not specified)22, 6/1225

1/381, 0/2010

0/381, 1/2010, 6/1225

Breast, infiltrating lobular

1/111, 0/610

10/111, 6/610

0/111, 0/610

0/111, 0/610

Thyroid neoplasms

0/55(24 were adenomas, 16 were medullary carcinomas, five were follicular carcinomas, and 10 were papillary carcinomas)10

54/55(24 were adenomas, 16 were medullary carcinomas, five were follicular carcinomas, and 10 were papillary carcinomas)10, 49/59(adenomas: 6/6 follicular and 6/6 oncocytic. carcinomas: 3/4 follicular, 8/8 papillary, 6/7 poorly differentiated, 4/6 Hurtle cell, 1/2 medullary, 0/4 anaplastic and 15/16 metastatic carcinomas)12

0/55(24 were adenomas, 16 were medullary carcinomas, five were follicular carcinomas, and 10 were papillary carcinomas)10

1/55(24 were adenomas, 16 were medullary carcinomas, five were follicular carcinomas, and 10 were papillary carcinomas)10, 10/59(adenomas: 0/6 follicular and 0/6 oncocytic. carcinomas: 1/4 follicular, 0/8 papillary, 1/7 poorly differentiated, 2/6 Hurtle cell, 1/2 medullary, 4/4 anaplastic and 1/16 metastatic carcinomas)12

Salivary gland

0/9(five were mixed tumors, two were Warthin’s tumors, and two were mucoepidermoid carcinomas)10, 0/ 15319, 6/8420

9/9(five were mixed tumors, two were Warthin’s tumors, and two were mucoepidermoid carcinomas)10, 153/15319, 78/8420

0/9(five were mixed tumors, two were Warthin’s tumors, and two were mucoepidermoid carcinomas)10, 0/15319, 0/8420

0/9(five were mixed tumors, two were Warthin’s tumors, and two were mucoepidermoid carcinomas)10, 0/15319, 0/8420

Thymoma

0/810, 0/3414

0/810, 28/3414

0/810, 0/3414

8/810, 6/3414

Oesophageal adenocarcinoma

1/30(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 11/11(oesophagus and oesophago-gastric junction)7

27/30(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 0/11(oesophagus and oesophago-gastric junction)7

1/30(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 0/11(oesophagus and oesophago-gastric junction)7

1/30(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 0/11(oesophagus and oesophago-gastric junction)7

Ovarian serous and endometrioid

0/191, 1/24(12 were serous papillary, four were clear cell, and eight were endometrioid)10, 2/11(endometrioid carcinomas: CK20 focally positive)15

19/191, 23/24(12 were serous papillary, four were clear cell, and eight were endometrioid)10, 9/11(endometrioid carcinomas)15

0/191, 0/24(12 were serous papillary, four were clear cell, and eight were endometrioid)10, 0/11(endometrioid carcinomas)15

0/191, 0/24(12 were serous papillary, four were clear cell, and eight were endometrioid)10, 0/11(endometrioid carcinomas)15

Endometrial

3/251, 0/1010

20/251, 10/1010

0/251, 0/1010

2/251, 0/1010

Cervix, squamous cell carcinoma

0/1510

13/1510

0/1510

2/1510

Predominantly CK7-/CK20+

CK7+/CK20+

CK7+/CK20-

CK7-/CK20+

CK7-/CK20-

Appendiceal adenocarcinoma

1/7(well differentiated)7, 8/23(These adenocarcinomas were metastatic to the ovary and included seven signet ring cell carcinoma of appendix: also 4 of 31 low grade adenomatous mucinous tumours)18

0/77, 0 /23(These adenocarcinomas were metastatic to the ovary and included seven signet ring cell carcinoma of appendix: also 0 of 31 low grade adenomatous mucinous tumours)18

6/7(well differentiated)7, 15 /23(These adenocarcinomas were metastatic to the ovary and included seven signet ring cell carcinoma of appendix: also 27 of 31 low grade adenomatous mucinous tumours)18

0/77, 0 /23(These adenocarcinomas were metastatic to the ovary and included seven signet ring cell carcinoma of appendix: also 0 of 31 low grade adenomatous mucinous tumours)18

Appendiceal goblet cell carcinoid

1/97, 4/6(appendiceal mixed goblet cell carcinoid / adenocarcinoma)7

0/97, 0/6(appendiceal mixed goblet cell carcinoid / adenocarcinoma)7

8/97, 2/6(appendiceal mixed goblet cell carcinoid / adenocarcinoma)7

0/97, 0/6(appendiceal mixed goblet cell carcinoid / adenocarcinoma)7

Colorectal adenocarcinoma (non-mucinous and mucinous): note that CK7 positivity may be more common in rectal carcinomas.

5/511, 16/772, 4/29(2 well/moderately differentiated and 2 poorly differentiated)7, 4/30(colorectal adenocarcinoma metastatic to lung)8, 1/2010, 12/225(Low grade 5/158, high grade 7/67 . Study used tissue arrays.)16, 0/11(Metastases to ovary . Study used tissue arrays.)16,  1/24(only focal expression of CK7)17, 3/28(These tumours were metastatic to the ovary and included one signet ring cell carcinoma of colorectum; all 3 were focally CK7+ and diffusely CK20+)18, 1/1224, 1/1425

0/511, 0/772, 1/29(poorly differentiated)7, 1/30(colorectal adenocarcinoma metastatic to lung)8, 0/2010, 9/225(Low grade 3/158, high grade 6/67 . Study used tissue arrays.)16, 0/11(Metastases to ovary . Study used tissue arrays.)16, 0/28(These tumours were metastatic to the ovary and included one signet ring cell carcinoma of colorectum)18

40/511, 59/772, 23/29(19 well/moderately differentiated and 4 poorly differentiated)7, 25/30(colorectal adenocarcinoma metastatic to lung)8, 19/2010, 3/3(metastatic to ovary)15, 153/225(Low grade 118/158, high grade 35/67 . Study used tissue arrays.)16, 10/11(Metastases to ovary . Study used tissue arrays.)16, 22/2417, 23/28(These tumours were metastatic to the ovary and included one signet ring cell carcinoma of colorectum; 17 were diffusely CK20+, 6 were focally CK20+)18, 11/1224, 11/1425

6/511, 2/772, 1/29(poorly differentiated)7, 0/2010, 51/225(Low grade 32/158, high grade 19/67 . Study used tissue arrays.)16, 1/11(Metastases to ovary . Study used tissue arrays.)16, 1/2417, 2/28(These tumours were metastatic to the ovary and included one signet ring cell carcinoma of colorectum)18, 2/1425

Merkel cell carcinoma

0/910

0/910

7/910

2/910

Predominantly CK7+/CK20+

CK7+/CK20+

CK7+/CK20-

CK7-/CK20+

CK7-/CK20-

Small intestinal adenocarcinoma

16/2417

8/2417

0/2417

0/2417

Ovarian mucinous

14/151, 10/18(invasive tumours[CK7 diffuse / CK20 focal; 4, CK7 diffuse / CK20 diffuse; 6]: also 29 of 35 borderline tumours [CK7 diffuse / CK20 focal; 14, CK7 focal / CK20 focal; 4, CK7 diffuse / CK20 diffuse; 10, CK7 focal / CK20 diffuse; 1])18

1/151, 8/18(invasive tumours[all diffusely CK7+]: also 4 of 35 borderline tumours[ CK7 diffuse; 3, focal 1])18

0/151, 0/18(invasive tumours: also 2 of 35 borderline tumours[CK20 diffuse; 1, focal; 1])18

0/151, 0/18(invasive tumours: also 0 of 35 borderline tumours)18

Endocervical adenocarcinoma

7/818

1/818

0/818

0/818

Bladder, transitional cell carcinoma

17/191, 12/264, 17/289, 6/24(well differentiated transitional cell carcinomas were more likely to be CK 20 positive)10

2/191, 12/264, 6/289, 15/2410

0/191, 1/289, 1/2410

0/191, 4/289, 2/2410

Cholangiocarcinoma

31/4813, 6/1410, 3/418, 5/14(intrahepatic cholangiocarcinoma metastatic to ovary; all cases showed diffuse staining for CK7 while only two cases showed diffuse positivity for CK20 and in only one case was it as strong as the CK7)21

15/4813, 7/1410, 0/418, 9/14(intrahepatic cholangiocarcinoma metastatic to ovary; 7 cases showed diffuse staining for CK7 and two cases only focal staining for CK7)21

2/4813, 0/1410, 1/418

0/4813, 1/1410, 0/418

Pancreatic adenocarcinoma

15/231, 8/1310, 11/14(These tumours were metastatic to the ovary )18

6/231, 4/1310, 3/14(These tumours were metastatic to the ovary )18

2/231, 0/1310, 0/14(These tumours were metastatic to the ovary )18

0/231, 1/1310, 0/14(These tumours were metastatic to the ovary )18

Predominantly CK7-/CK20-

CK7+/CK20+

CK7+/CK20-

CK7-/CK20+

CK7-/CK20-

Head and neck, squamous cell carcinoma

0/3010

8/3010

2/3010

20/3010

Lung, squamous cell

0/121, 0/1510

0/121, 7/15(this figure is taken from table 3 but is contradicted by their table 1 and the text of their results. It is possible that this entry should read "0/15")10, 7/8(using cell blocks)23

1/121, 0/1510

11/121, 8/15(this figure is taken from table 3 but is contradicted by their table 1 and the text of their results. It is possible that this entry should read "15/15)10, 1/8(using cell blocks)23

Lung, small cell

0/111, 0/710

2/111, 3/710, 4/5(using cell blocks)23

0/111, 0/710

9/111, 4/710, 1/5(using cell blocks)23

Lung, carcinoid

0/910, 0/1611

2/910, 9/1611

0/910, 0/1611

7/910, 7/1611

Oesophagus, squamous cell carcinoma

0/1410

3/1410

0/1410

11/1410

Gastrointestinal carcinoid

0/1510

2/1510

1/1510

12/1510

Colonic high grade neuroendocrine carcinoma

0/57

0/57

0/57

5/5(2 small cell carcinomas, 2 large cell neuroendocrine carcinomas, 1 mixed small cell / large cell neuroendocrine carcinoma)7

Hepatocellular carcinoma

2/301, 0/1110

5/301, 1/1110

0/301, 1/1110

23/301, 9/1110

Renal cell carcinoma

0/171, 0/1910

4/171, 2/1910

1/171, 0/1910

12/171, 17/1910

Prostatic adenocarcinoma

1/131, 1/599, 0/1810

1/131, 5/599, 0/1810

3/131, 5/599, 0/1810

8/131, 48/599, 18/1810

Germ cell tumour

0/14(six were embryonal carcinomas, four were seminomas, three were teratomas, and one was a yolk sac tumor)10

1/14(six were embryonal carcinomas, four were seminomas, three were teratomas, and one was a yolk sac tumor)10

0/14(six were embryonal carcinomas, four were seminomas, three were teratomas, and one was a yolk sac tumor)10

13/14(six were embryonal carcinomas, four were seminomas, three were teratomas, and one was a yolk sac tumor)10

Epithelioid sarcoma

0/1210

0/1210

0/1210

12/1210

Adrenal cortical tumour

0/1010

0/1010

0/1010

10/1010

Variable

CK7+/CK20+

CK7+/CK20-

CK7-/CK20+

CK7-/CK20-

Gastric adenocarcinoma

11/291, 5/14(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 27/38(5 well/moderately differentiated and 22 poorly differentiated)7, 1/810, 75/289(Intestinal type 26/112, diffuse type 49/177 . Study used tissue arrays.)16, 16/43(Metastases to ovary . Study used tissue arrays.)16, 4/518

5/291, 3/14(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 8/38(3 well/moderately differentiated and 5 poorly differentiated)7, 2/810, 132/289(Intestinal type 52/112, diffuse type 80/177 . Study used tissue arrays.)16, 18/43(Metastases to ovary . Study used tissue arrays.)16, 0/518

10/291, 3/14(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 3/38(all 3 poorly differentiated)7, 3/810, 42/289(Intestinal type 19/112, diffuse type 23/177 . Study used tissue arrays.)16, 8/43(Metastases to ovary . Study used tissue arrays.)16, 1/518

3/291, 3/14(Tumours were considered positive if at least 50% of cells showed at least moderate immunoreactivity.)3, 0/387, 2/810, 40/289(Intestinal type 15/112, diffuse type 25/177 . Study used tissue arrays.)16, 1/43(Metastases to ovary . Study used tissue arrays.)16, 0/518