Mammaglobin A, secretoglobin, family 2A, member 2 SCGB2A2

The mammaglobin gene encodes a 10 kDa protein which is a member of a family of secretory proteins which includes uteroglobin. The gene has ben mapped to chromosome 11q12.3-q13.12. Mammaglobin (MGB1) and mammaglobin-2 are highly homogenous proteins, both expressed by breast tissue.

Immunohistochemical expression

An antibody against mammaglobin is effective on formalin-foxed paraffin-embedded sections. Staining is cytoplasmic. Cross reaction with uteroglobin may account for the positivity in endometrioid carcinomas2.

Normal breast tissue shows some positive and some negative lobules1.

Breast

in situ ductal carcinoma

4/111

ductal carcinoma

105/2141, 9/142, 50/91 (detail)7

lobular carcinoma

4/51, 10/142, 21/247

mucinous carcinoma

1/41

medullary carcinoma

0/11

metaplastic carcinoma

0/21

small cell carcinoma

0/11

luminal cell subtype

81/1561

HER2 subtype

26/491, 5/72

basal cell subtype

5/251

negative subtype

2/81

Total

114/2381

breast carcinoma metastatic to lung

6/101

breast carcinoma metastatic to pleura

11/203

Lung

adenocarcinoma

0/791, 0/182, 0/1587

squamous cell carcinoma

0/171, 0/272, 0/397

other non small cell carcinomas

0/101

metastatic to pleura

0/323

Skin

melanoma

5/802

sweat gland carcinoma

4/102

squamous cell carcinoma

0/142

basal cell carcinoma

0/132

DFSP

0/92

malignant Schwannoma

0/32

fibrosarcoma

0/12

leiomyosarcoma

0/12

sebaceous carcinoma

0/12

Salivary gland

pleomorphic adenoma

3/41, 1/22

adenoid cystic carcinoma

1/41, 0/22

acinic cell tumour

0/12

mucoepidermoid carcinoma

1/11, 1/32

salivary duct carcinoma

1/21

adenocarcinoma NOS

2/32

mucinous carcinoma

0/12

adenosquamous carcinoma

0/12

basal cell

0/22

epithelial-myoepithelial carcinoma

1/22

undifferentiated carcinoma

0/22

sarcomatoid carcinoma

0/12

malignant myoepithelioma

0/12

MFH

0/12

Warthin tumour

0/12

Total

6/111, 5/252

Head and neck

squamous carcinoma

0/201

Oesophagus

adenocarcinoma

0/11

squamous cell carcinoma

0/81

Stomach

adenocarcinoma

0/191, 0/352

carcinoid

0/22

Colon

adenocarcinoma

0/161, 0/392

Pancreas

adenocarcinoma

0/31, 1/672

Kidney

clear cell

0/372

undifferentiated

0/22

Bladder

urothelial

0/372

adenocarcinoma

0/22

squamous cell carcinoma

0/12

Uterine cervix

squamous cell carcinoma

0/101, 0/412

in situ adenocarcinoma

5/112

adenocarcinoma

0/41, 0/92

Endometrium

adenocarcinoma

1/91, 2/141, 23/592, 0/43

invasive mole

0/12

Ovary

adenoma

0/31

papillary serous carcinoma

1/382

mucinous

0/12

clear cell

0/12

adenocarcinoma

0/101, 0/93

Lymphoma

0/1(renal)2

Primary carcinomas and lymph node metastases show a high degree of concordance2. Staining is not a function of tumour grade2. One study showed a concordance with steroid receptor positivity1, a second did not2.

Mammaglobin has a greater sensitivity than has GCDFP15. The patchiness of staining would account for the lesser sensitivity of TMAs.

		Mammaglobin
		positive	equivocal(focal and/or weak staining)	negative	total
GCDFP15	positive	19(whole tissue sections)2, 3(tissue microarray)2	3(whole tissue sections)2, 0(tissue microarray) 2	2(whole tissue sections)2, 1(tissue microarray) 2	24(whole tissue sections)2, 4(tissue microarray) 2
	equivocal(focal and/or weak staining)	17(whole tissue sections)2, 1(tissue microarray)2	8(whole tissue sections)2, 1(tissue microarray) 2	0(whole tissue sections)2, 6(tissue microarray) 2	25(whole tissue sections)2, 8(tissue microarray) 2
	negative	5(whole tissue sections)2, 22(tissue microarray)2	2(whole tissue sections)2, 10(tissue microarray) 2	2(whole tissue sections)2, 19(tissue microarray) 2	9(whole tissue sections)2, 51(tissue microarray) 2
	total	41(whole tissue sections)2, 26(tissue microarray) 2	13(whole tissue sections)2, 11(tissue microarray) 2	4(whole tissue sections)2, 26(tissue microarray) 2	58(whole tissue sections)2, 63(tissue microarray) 2

	BRST-1	GCDFP-1	mammaglobin
Breast carcinoma	53/704	31/704	59/704
Lung carcinoma	11/304	1/304	5/304
Gastric carcinoma	6/304	0/304	4/304
Colorectal carcinoma	1/254	0/254	3/254
hepatobiliary carcinoma	6/204	0/204	2/204
Thyroid carcinoma	1/104	0/104	2/104
Transitional cell carcinoma	5/104	0/104	2/104
Ovarian and uterine carcinoma	5/104	0/104	2/104
Salivary gland carcinoma	2/54	2/54	1/54

Positivity for mammaglobin correlates with disease-free time but is not significant on multivariate analysis1.

Diagnostic utility

Mammaglobin expression is a sensitive molecular marker for the detection of micrometastases in tumor-draining lymph nodes by PCR5.
Identification of adenocarcinomas arising from the breast. Lung carcinomas are reliably completely negative1.
Possibly in the differentiation of endometrial (positive) from endocervical (negative) carcinoma2.

References

1 Sasaki E, Tsunoda N, Hatanaka Y, et al. Breast-specific expression of MGB1/mammaglobin: an examination of 480 tumors from various organs and clinicopathological analysis of MGB1-positive breast cancers. Mod Pathol 2007; 20:208-14

2 Bhargava R, Beriwal S,Dabbs DJ Mammaglobin vs GCDFP-15: an immunohistologic validation survey for sensitivity and specificity. Am J Clin Pathol 2007; 127:103-13

3 Ciampa A, Fanger G, Khan A, et al. Mammaglobin and CRxA-01 in pleural effusion cytology: potential utility of distinguishing metastatic breast carcinomas from other cytokeratin 7-positive/cytokeratin 20-negative carcinomas. Cancer 2004; 102:368-72 FULL TEXT

4 Han JH, Kang Y, Shin HC, et al. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med 2003; 127:1330-4 FULL TEXT

5 Kataoka A, Mori M, Sadanaga N, et al. RT-PCR detection of breast cancer cells in sentinel lymph modes. Int J Oncol 2000; 16:1147-52

6 Zafrakas M, Petschke B, Donner A, et al. Expression analysis of mammaglobin A (SCGB2A2) and lipophilin B (SCGB1D2) in more than 300 human tumors and matching normal tissues reveals their co-expression in gynecologic malignancies. BMC Cancer 2006; 6:88 FULL TEXT

7 Yang M, Nonaka D. A study of immunohistochemical differential expression in pulmonary and mammary carcinomas. Mod Pathol. 2010 May;23(5):654-61. (with corrections to the paper provided by author)

This page last revised 10.5.2010.

Mammaglobin A, secretoglobin, family 2A, member 2  SCGB2A2

The mammaglobin gene encodes a 10 kDa protein which is a member of a family of secretory proteins which includes uteroglobin. The gene has ben mapped to chromosome 11q12.3-q13.12. Mammaglobin (MGB1) and mammaglobin-2 are highly homogenous proteins, both expressed by breast tissue.

Immunohistochemical expression

Primary carcinomas and lymph node metastases show a high degree of concordance2. Staining is not a function of tumour grade2. One study showed a concordance with steroid receptor positivity1, a second did not2.

Mammaglobin has a greater sensitivity than has GCDFP15. The patchiness of staining would account for the lesser sensitivity of TMAs.

Positivity for mammaglobin correlates with disease-free time but is not significant on multivariate analysis1.

Diagnostic utility

Mammaglobin A, secretoglobin, family 2A, member 2 SCGB2A2